Time-Resolved Metabolic Footprinting for Nonlinear Modeling of Bacterial Substrate Utilization
نویسندگان
چکیده
منابع مشابه
Time-resolved metabolic footprinting for nonlinear modeling of bacterial substrate utilization.
Untargeted profiling of small-molecule metabolites from microbial culture supernatants (metabolic footprinting) has great potential as a phenotyping tool. We used time-resolved metabolic footprinting to compare one Escherichia coli and three Pseudomonas aeruginosa strains growing on complex media and show that considering metabolite changes over the whole course of growth provides much more inf...
متن کاملTime-resolved footprinting for the study of the structural dynamics of DNA-protein interactions.
Transcription is often regulated at the level of initiation by the presence of transcription factors or nucleoid proteins or by changing concentrations of metabolites. These can influence the kinetic properties and/or structures of the intermediate RNA polymerase-DNA complexes in the pathway. Time-resolved footprinting techniques combine the high temporal resolution of a stopped-flow apparatus ...
متن کاملDeterminants of Brain Cell Metabolic Phenotypes and Energy Substrate Utilization Unraveled with a Modeling Approach
Although all brain cells bear in principle a comparable potential in terms of energetics, in reality they exhibit different metabolic profiles. The specific biochemical characteristics explaining such disparities and their relative importance are largely unknown. Using a modeling approach, we show that modifying the kinetic parameters of pyruvate dehydrogenase and mitochondrial NADH shuttling w...
متن کاملMetabolic footprinting of mutant libraries to map metabolite utilization to genotype.
The discrepancy between the pace of sequencing and functional characterization of genomes is a major challenge in understanding complex microbial metabolic processes and metabolic interactions in the environment. Here, we identified and validated genes related to the utilization of specific metabolites in bacteria by profiling metabolite utilization in libraries of mutant strains. Untargeted ma...
متن کاملModeling Time Resolved Light Propagation Inside a Realistic Human Head Model
Background: Near infrared spectroscopy imaging is one of the new techniques used for investigating structural and functionality of different body tissues. This is done by injecting light into the medium and measuring the photon intensity at the surface of the tissue.Method: In this paper the different medical applications, various imaging and simulation techniques of NIRS imaging is described. ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 2009
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.01742-08